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Journal of the Korean Academy of Rehabilitation Medicine 2003;27(2):232-239.
Effects of Exercise and Steroid on Apoptosis in Skeletal Muscle of Mdx Mouse.
Bang, Moon Suk , Lim, Jung Hun , Kim, Dai Youl
Department of Rehabilitation Medicine, Seoul National University College of Medicine, Korea. rheumagene@hotmail.com
운동 및 스테로이드가 근육병 생쥐 근육의 아포프토시스에 미치는 영향
방문석, 임정훈, 김대열
서울대학교 의과대학 재활의학교실
To investigate the effect of exercise and steroid to the muscle of animal model of Duchenne muscular dystrophy.

We used 15 mdx and 15 control mice. To grade exercise loading, control and mdx mice were divided into free-living, exercise and immobilization groups. Free-living and exercise groups were further divided into steroid-treated and sham-treated groups to evaluate the effect of steroid administration. We measured the apoptotic changes using in situ DNA nick-end labling (TUNEL), DNA fragmentation assay and western blots for Bcl-2 and BAX.

With TUNEL method, the largest number of myonuclei became positive in sham-treated exercise group while apoptosis was significantly reduced in steroid-treated exercise group in mdx mice. Steroid-treated free-living group showed higher rate of apoptotic change than sham-treated free-living group. With western blots for Bcl-2 and BAX, the value of BAX/Bcl-2 ratio was highest in sham-treated exercise group and among free living mdx mice, it was higher in steroid-treated group than sham-treated one.

Apoptosis can be minimized in free living condition while exercise loading or immobilization can cause apoptotic change in muscular dystrophy animal model. Steroid administration induces apoptosis in free living muscle and it alleviates apoptotic damage caused by exercise loading in mdx mice. (J Korean Acad Rehab Med 2003; 27: 232-239)

Key Words: Exercise, Steroid, Apoptosis, Mdx mouse, Muscular dystrophy


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