| Effect of Low Power Laser Irradiation on the Proliferation and Differentiation of PC12 Nerve Cells. |
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Joo, Hyung Wook , Lee, Seong Jae , Hyun, Jung Keun , Kim, Byung Hee , Park, Won Beom , Lee, Chang Ho , Kwon, Bum Sun |
1Department of Rehabilitation Medicine, Dankook University College of Medicine.
2Department of Physical Medicine & Rehabilitation, Donngguk University College of Medicine. bskwon@dankook.kr |
| 저출력 레이저 조사가 PC12 신경 세포 증식과 분화에 미치는 영향 |
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주형욱, 이성재, 현정근, 김병희, 박원범, 이창호, 권범선1 |
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단국대학교 의과대학 재활의학교실, 1동국대학교 의과대학 재활의학교실 |
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| Abstract |
Objective
We examined the effect of low power laser irradiation (LPLI) on the proliferation and differentiation of PC12 nerve cells.
Method
After seeding 4⁓105 PC12 nerve cells each in the 24 well-culture dishes, we cultured them for 6 days with RPMI1640 media. LPLI (650 nm, 5 mW, 5 sec) was applied for 1 day, 2 days and 3 days (1, 2 and 3-day-LPLI groups) consecutively. For the degree of proliferation of PC12 nerve cells, we compared the total cell number at 6th day after LPLI by MTT cell proliferation assay. For the degree of differentiation, we compared the length of neurite out- growth and the expression of RT97 at 6th day after adding nerve growth factor on each group.
Results
The total cell numbers were increased significantly after LPLI, but those increments were not significant among 1, 2, and 3-day-LPLI groups. The numbers of the differentiated PC12 nerve cells and the expressions of RT97 were diminished serially according to the number of days of LPLI.
Conclusion
We conclude that LPLI increased the proliferation and decreased the differentiation of PC12 nerve cells. We could suggest that single or short-term use of LPLI on the injured nerve should be helpful for enhancing the neural regeneration in vivo. (J Korean Acad Rehab Med 2005; 29: 419-423) |
| Key Words:
Low power laser, Neural regeneration, PC12 nerve cell, RT97 |
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