Method: Rectus femoris muscles of rats were divided into 4 groups according to conditioned ischemic stresses as followings: sham-operated control group; 2 hour ischemia group by clamping the proximal femoral vessel; ischemia- reperfusion group by 30 minutes of reperfusion following 2 hours of ischemia; ischemia-reperfusion-electrical nerve stimulation group in which current was applied at the femoral nerve during 30 min of reperfusion period to induce repetitive muscle contraction. Either genistein-5% dimethyl sulfoxide (DMSO) or DMSO was injected 2 hours before ischemic induction. NF-κB activities were analysed by electrophoretic mobility shift assay.

Results: NF-κB activities pretreated with DMSO were significantly increased in ischemia, ischemia-reperfusion and ischemia-reperfusion-electrical nerve stimulation group compared with those of control group and in ischemia- reperfusion-electrical nerve stimulation group compared with those of ischemia group. Muscle edema was increased in ischemia- reperfusion-electrical nerve stimulation group. But, NF-κB activities and muscle edema pretreated with genistein were attenuated in ischemia-reperfusion-electrical nerve stimulation group compared with those pretreated with DMSO alone.

Conclusion: NF-κB activities reflect the severity of ischemic stresses. Pretreatment with genistein can attenuate NF-κB activities and muscle edema in ischemic stress of rat skeletal muscle. (J Korean Acad Rehab Med 2003; 27: 990-996)

Keywords :NF-κB, Ischemic stress, Electrical stimulation, Genistein, Skeletal muscle