Muscle Fiber Conduction Velocity and Histopathologic Findings after Intramuscular Injection with 7% Phenol Solution in Rat. |
Jeong, Sang Wook , Park, Hee Seok , Kim, Ghi Chan , Jeong, Ho Joong , Jang, Hee Kyeong |
1Department of Rehabilitation Medicine, Kosin Medical College, Pusan, Korea. 2Department of Anatomic Pathology, Kosin Medical College, Pusan, Korea. |
흰쥐에서 7% 페놀용액 근주후 병리 소견 및 근섬유 전도속도 검사 |
정상욱, 박희석, 김기찬, 정호중, 장희경1 |
고신대학교 의학부 재활의학과교실, 1해부병리학과교실 |
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Abstract |
Objective To investigate the muscle fiber conduction velocity and histopathologic changes in skeletal muscle after 7% phenol solutions intramuscular injection. Method Forty-five Sprague Dawley rats were divided into two groups: Experimental group, 30; Control group, 15. Experimental and control groups were injected with 0.1 cc of 7% phenol solution and 0.9% saline in right gastrocnemius, respectively. The histopathologic findings and muscle fiber conduction velocity were evaluated at 0, 1, 5, 14, and 28 days after each injection. Results In the light microscopic examination of experimental groups, vacuolar change, atrophy of myofibers, and intermyofiber cell proliferation were shown. The expression of synaptophysin began to be seen at 5 days and S-100 protein was increased 14 days after injection. In the electron microscopic examination of experimental groups, vacuolar change and denuded postsynaptic membrane were shown. In muscle fiber conduction study of experimental groups, the mean velocity were significantly slowed at 1, 5, 14, and 28 days after injection than those of control groups (p<0.05). Conclusion On the basis of histopathologic findings, it is presumed that the mechanisms of paralysis following intramuscular injection of 7% phenol solutions were both myogenic and neurogenic. The slowing of muscle fiber conduction velocity tended to be related with the histopathologic changes of skeletal muscles after intramuscular injection with 7% phenol solution. |
Key Words:
7% Phenol solution, Histopathologic changes, Muscle fiber conduction velocity |
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