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Journal of the Korean Academy of Rehabilitation Medicine 2001;25(1):140-149.
In Vitro Effects of Ultrasound on Fibroblast Like Synoviocytes from Rheumatoid Arthritis.
Kim, Hyeon Sook , Han, Tai Ryoon , Koh, Eun Mi
1Department of Physical Medicine and Rehabilitation, Samsung Medical Center, Sungkyunkwan University School of Medicine.
2Department of Internal Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine.
3Department of Rehabilitation Medicine, Seoul National University College of Medicine.
초음파가 류마티스 관절염의 섬유모세포양 활막세포에 미치는 영향
김현숙, 한태륜2, 고은미1
성균관대학교 의과대학 삼성서울병원 재활의학교실, 1내과학교실, 2서울대학교 의과대학 재활의학교실
Abstract

Objective
Ultrasound has been therapeutically applied for pain control in rheumatoid arthritis although little physiologic effects of sonication on rheumatoid tissue were known. This investigation was conducted to determine the effects of sonication on the cell proliferation and matrix metalloproteinase (MMP) production of cultured fibroblast like synoviocytes (FLS) derived from synovial tissues of rheumatoid arthritis.


Method
Pulsed ultrasound (1.0 MHZ, 20 msec on, 80 msec off) with varying intensities (0, 0.1, 0.25, 0.5, 0.75, 1.0 W/cm2) was applied to experimental cell groups growing as monolayers in culture plates for varying durations (0, 30, 90, 180 seconds) in the presence and absence of interleukin-1β (IL-1β).


Results
There were no significant differences in thymidine incorporation between 0, 30, 90 and 180 second sonication groups with 0.5 W/cm2 after 1 day and 2 days. There were no significant differences in thymidine incorporation between 0, 0.1, 0.25, 0.5, 0.75, 1.0 W/cm2 sonication groups 1 day and 2 days after 90 second sonication. There were significant increase in MMP-1 (p=0.025) and MMP-3 production (p=0.000) of FLS after sonication in the absence of IL-1β but there were no significant differences in MMP-1 and MMP-3 production in the presence of IL-1β. And MMP-1 and MMP-3 production were increased significantly in the presence of IL-1β but not than in the absence of IL-1β.


Conclusion
While comparisons made between a limited number of FLS cell lines must be open to question, the overall consistency of the findings suggest sonication with nonthermal effect is not the contraindication in rheumatoid arthritis treatment but further study is needed in vivo in animal and in clinical studies.

Key Words: Synoviocyte (fibroblast like synoviocyte), Stromelysin, Sonication, Rheumatoid arthritis, Synoviocyte (fibroblast like synoviocyte), Collagenase, Stromelysin


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