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Journal of the Korean Academy of Rehabilitation Medicine 2001;25(6):1074-1080.
Expression of Heat Shock Protein 70 by Heat Stimulation in Rabbit Chondrocytes and Articular Cartilages.
Lee, Kang Hee , Ahn, Sang Ho , Jang, Seong Ho , Baek, Suk Hwan
1Department of Rehabilitation Medicine, Yeungnam University College of Medicine, Korea.
2Department of Biochemistry, Yeungnam University College of Medicine, Korea.
3Department of Molecular Biology, Yeungnam University College of Medicine, Korea.
토끼 연골 세포 및 조직에서 열자극에 의한 열충격 단백질 70의 발현
이강희, 안상호, 장성호, 백석환1
영남대학교 의과대학 재활의학과, 1생화학, 분자생물학교실
Abstract

Objective
Heat therapy is one of physical therapies used most commonly in chronic osteoarthritis. The therapeutic effects of heat therapy might be attributed to induce heat shock proteins in heat-stimulated cells and tissues and therefore, to inhibit cellular damages due to inflammation. In order to investigate preliminarily the therapeutic effects of heat therapy, Hsp(heat shock protein) 70 expressions by heat stimulation were measured in cultured chondrocytes and knee joint cartilages of rabbits.


Method
Five rabbits were used in total in this study. Three rabbits were used for chodrocytes culture and two rabbits were in vivo study. Chondrocytes were cultured from knee cartilages of three rabbits and treated at 33oC, 37oC, and 42oC for 20 min. In order to clarify whether heat therapy using ultrasonification can induce Hsp 70 expression in cartilage tissues, right knees of rabbits were heat-stimulated by ultrasonification for 20 min and their left knees were untreated. After 2 hours, cultured chondrocytes and cartilages were prepared and Hsp 70 expression was also observed by Western blot analysis.


Results
Hsp 70 expression was increased 1.48 folds in 42oC treated cells compared to in 37oC treated cells. The heat-stimulated cartilages showed 1.65 fold increases in Hsp 70 expression compared to the unstimulated cartilages.


Conclusion
Hsp 70 expressions were increased by heat stimulation in cultured chondrocytes as well as in cartilage tissues.

Key Words: Heat shock protein, Chondrocytes, Cartilage
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