| Expression of Caveolin-3 in the Muscle Cell and Tissue. |
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Kwon, Bum Sun , Lee, Seong Jae , Hyun, Jung Keun , Jun, Dong Jin , Joo, Hyung Wook , Kim, Byung Hee , Shin, Dong Hoon |
1Department of Rehabilitation Medicine, Dankook University College of Medicine, Korea. bskwon@dankook.ac.kr
2Department of Anatomy, Seoul National University College of Medicine, Korea. |
| 근육 세포와 조직에서 Caveolin-3의 발현 양상 |
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권범선, 이성재, 현정근, 전동진, 주형욱, 김병희, 신동훈1 |
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단국대학교 의과대학 재활의학교실, 1서울대학교 의과대학 해부학교실 |
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| Abstract |
Objective
Caveolae are the microdomain of the plasma membrane that have been implicated in signal transduction and caveolin is a principal component of the caveolae. Caveolin-3, a family of caveolin related protein, is expressed only in muscle tissue. Here we examined the expression of caveolin-3 in the course of myobalst differentiation and within the muscle tissue.
Method
L6 cell, rat skeletal myoblast, was cultured in the low mitogen medium and caveolin-3 expression was observed both by immunocytochemistry and western blot analysis. Localization of caveolin-3 within the muscle tissue was investigated and compared to that of dystrophin.
Results
While caveolin-3 was not expressed in the proliferating myolast, caveolin-3 was expressed in the differentiated myoblast. Caveolin-3 and dystrophin were co-expressed in the membrane of muscle tissue and integrated density of caveolin-3 was elevated in the area of muscle injury. In the Duchenne muscular dystrophy, caveolin-3 was expressed in the membrane of muscle tissue, but dystrophin was not.
Conclusion
Caveolin-3 was induced during the myobalst differentiation and its expression was increased during the muscle regeneration. Caveolin-3 was physically associated with dystrophin as a complex, but not absolutely required for the biogenesis of dystrophin complex. (J Korean Acad Rehab Med 2003; 27: 382-387)
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| Key Words:
Caveolin-3, Dystrophin, Duchenne muscular dystrophy, Myoblast, Skeletal muscle |
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