Expression of Caveolin-3 in the Myelin Sheath of Peripheral Nerve.

, Kwon, Bum Sun

Original Article

Journal of the Korean Academy of Rehabilitation Medicine 2006;30(4):353-356.

Kwon, Bum Sun

Department of Rehabilitation Medicine, Dongguk University College of Medicine, Korea. bskwon@duih.org

말초신경 수초에서 카베올린-3의 발현

권범선

동국대학교 의과대학 재활의학교실

Abstract

Objective
To exhibit the caveolin-3 immunoreactivities (IRs) in the peripheral nerve, which was previously known to be present only within the muscle and to be a causative agent of myopathy
Method
The sciatic nerves of the rat were removed after the perfusion and frozen after cryoprotection by sucrose. The tissue specimens were cut on cryostat and immunostained with anti-caveolin-3 and growth associated protein-43 (GAP-43) antibodies. The sections were observed with a fluorescence microscope.
Results
We detected caveolin-3 IRs in myelin sheath of the peripheral nerves, while GAP IRs were detected in the axon. Caveolin-3 IRs were active in the rat of postnatal 1 week, but they were reduced in the rat of postnatal 3 week and disappeared in that of 5 week.
Conclusion
We detected caveolin-3 IRs in the myelin sheath of peripheral nerve. Caveolin-3 might play roles in the early myelination of peripheral nerve. (J Korean Acad Rehab Med 2006; 30: 353-356)

Key Words: Caveolin-3, Immunocytochemistry, Peripheral nerve, Regeneration