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Journal of the Korean Academy of Rehabilitation Medicine 2009;33(2):186-193.
Phosphorylation of Extracellular Signal-Regulated Kinase1/2 and Caldesmon in Rat Colon by Electrical Stimulation to Sacral Plexus Following Spinal Cord Injury.
Joo, Min Cheol , Han, Yong Jae , Kim, Tae Jin , Yoo, Su Jin , Kim, Yong Sung , Lee, Moon Young
1Department of Rehabilitation Medicine, Wonkwang University School of Medicine, Korea.
2Department of Emergency Medicine, Wonkwang University School of Medicine, Korea.
3Department of Internal Medicine and Digestive Disease Research Institute, Wonkwang University School of Medicine, Korea.
4Department of Physiology and Institute of Wonkwang Medical Science, Wonkwang University School of Medicine, Korea. lmy6774@wku.ac.kr
전기자극이 척수손상 대장의 pCaldesmon 발현에 미치는 영향
주민철,한용재1,김태진,유수진1,김용성2,이문영3
원광대학교 의과대학 재활의학교실, 1응급의학교실, 2내과학교실 및 소화기질환연구소, 3생리학교실 및 원광의과학연구소
Abstract
Objective
To find out whether electrical stimulation affects intracellular signaling mechanisms that link the biochemical and mechanical events of smooth muscle contraction.
Method
A total of 31 adult Sprague-Dawley female rats were divided into 3 groups: control group, spinal cord injury (SCI) only group, and spinal cord injury with elec-trical stimulation (SCI+ES) group. Complete spinal cord transec-tion was performed surgically at T10 cord level. The electrode for electrical stimulation was implanted into sacral spinal cord region (S2-4). Electrical stimulation was applied 4 hours per day from the day of operation.
Results
In SCI+ES group, the weights of fecal pellet were significantly higher from the 3rd day of post-operation to the 6th day than the SCI only group. The numbers of pERK 1/2 immunoreactive cells significantly increased in all colon segments of the SCI+ES group but had decreased in the SCI only group. Western blot showed the stronger bands of phosphorylated ERK1/2 in all colon segments and also phosphorylated caldesmon in mid or distal colon segments in the SCI+ES group.
Conclusion
These results suggest that electrical stimulation to sacral plexus region activate phosphorylation of ERK1/2 and caldesmon which leads to improvement of bowel fun-ction by promotion of secretion or motility in the colon. (J Korean Acad Rehab Med 2009; 33: 186-193)
Key Words: Spinal cord injury, Electrical stimulation, Extracellular signal-regulated kinase, pCaldesmon, Gastrointestinal motility, Fecal pellet


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