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Journal of the Korean Academy of Rehabilitation Medicine 2011;35(1):8-13.
Myogenic Differentiation of Human Adipose-Derived Stem Cells.
Park, Yoon Ghil , Baek, Ah Mi , Do, Byung Rok , Choi, Jung Hwa , Kim, Sun Do
1Department of Rehabilitation Medicine, Yonsei University College of Medicine, Seoul 135-720, Korea.
2Rehabilitation Institute of Muscular Disease, Yonsei University College of Medicine, Seoul 135-720, Korea.
3Hurim Biocell, Seoul 157-810, Korea.
4Department of Rehabilitation Medicine, Yonsei University College of Medicine, Seoul 120-752, Korea. jhchoi1216@yuhs.ac
Abstract
Objective
Cell therapy has been extensively studied as a gene complementation approach in muscular dystrophy including Duchenne muscular dystrophy (DMD), and adipose tissue has recently been identified as a uniquely abundant and adequately accessible source of pluripotent cells. In the present work, we investigated myogenic potentials of adipose-derived stem cells (ADSCs) depending on culture media and isolation with using surface markers. Method Human ADSCs were obtained by liposuction and cultured in two different media; control and myogenic media. In addition we attempted to isolate ADSCs by utilizing surface markers: CD45 and CD133. The following observations were made to evaluate myogenic differentiation as the expression of myogenic regulatory factors (MyoD, Myf-5 and Myf-6) and desmin by RT-PCR and immunoflurescence study.
Results
Conversion of ADSCs to myogenic phenotype was observed by indirect immunoflurescence study of MyoD and Myf-5 in regardless of media type and isolation method. In addition mRNA of MyoD and Myf-5 were positive in both culture media, and there were no differences of MyoD and Myf-5 responses between CD45− and CD45−CD133− ADSCs. However, secondary myogenic regulatory factor (Myf-6) was not expressed constantly, and desmin were negative in all cultural condition. Conclusion Our findings suggest that human ADSCs might have myogenic potentials. However, further studies are needed to express the secondary myogenic regulatory factors and proteins in myoblasts.
Key Words: Muscular dystrophy, Stem cell, Differentiation, Myogenic regulatory factor, Desmin
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